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Abstract

The present study aims to investigate the inhibitory effect of shiitake mushrooms on some common fungi found in the soil of Mosul City. The fungal extract from Shiitake was prepared using ethanol or water. Different concentrations of the extract were prepared to analyze the antifungal properties of the mushroom by first making a stock solution and then adding specific amounts to the plates containing (Potato Dextrose Agar) PDA medium to achieve the desired concentrations. The active components found in Shiitake were pinpointed through the use of HPLC. The findings revealed how the aqueous extract of Shiitake affected Trichoderma sp. Penicillium sp. with the impact intensifying as the extract concentration levels rose. In the case of Rhizopus sp. and Aspergillus sp. the influence was noticeable, at a concentration of 0.5 units. Increased with concentrations. Furthermore the ethanolic extract exhibited efficacy against Trichoderma sp., Penicillium sp. and Rhizopus sp. At concentration levels leading to a decrease in growth diameter as the concentration increased. As for Aspergillus sp. its impact was more pronounced at concentrations. Analysis using HPLC revealed that phenolic compounds such as Chlorogenic Acid, Quercetin, Caffeic Acid, Lutine and Vanillic Acid were identified, along with their retention times and concentrations in mg/g for both types of extracts. Notably Vanillic acid and Caffeic acid demonstrated effectiveness compared to compounds present in Shiitake mushrooms. In conclusion, it is evident that shiitake mushrooms contain levels of compounds particularly vanillic acid and caffeic acid, which play a vital role, in combating fungal infections.

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Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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