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Abstract

Background: Hematopoietic stem cells (HSC) are cells that can differentiate according to the tissue they are lying in. The transplantation of such cells is either autologous or allogeneic. Autologous storage is required until the patient finishes his intensive chemotherapy. Whilst allogenic storage is required until finding the suitable matched recipient for this cellular therapy. Storage is in deep frozen state (–80° C) with cryoprotectant compound. Dimethyl sulphoxide (DMSO) has been used as cryoprotectant due to its electrophilic property that makes H-bond between DMSO and H2O, which is 2.6 times stronger than the H bond between two water molecules. As a result, minimal concentration of DMSO prevents formation of water crystals during freezing storage of cells. Objectives: Assessing the cytotoxicity of DMSO on stored HSC, by determining the cells' viability after adding DMSO with concentration of 10%, after deep-freezing in specialized deep freeze at (–80° C), then in ordinary freeze at (–20° C), in room temperature, in ordinary refrigerator at (+4° C), and lastly in water bath incubation at (+37 ° C), each for 24 hours. Methods: Using trypan blue dye, the vitality was assessed by counting the alive cells (uncolored), and dead cells (blue colored). Results: Cells retain high membrane integrity when kept at (–80° C) and (–20° C), but when exposed to room temperatures (+22° C) and physiological temperature (+37° C), this will rapidly decrease cell viability. Conclusions: Although prolonged incubation time further decreases viability, the essential driver of DMSO cytotoxicity is elevated temperature. The post-thaw cytotoxicity of 10% DMSO cryopreserved peripheral HSCs is greatly temperature dependent.

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Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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